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Background: Osteopontin, OPN （designated Nephropontin or Secreted phosphoprotein 1, SPP-1 ）. Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.

Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. Extensively phosphorylated on serine residues.

Belongs to the osteopontin family.

Specificity : · anti-OPN is a rabbit polyclonal antybody

· epitope mapping near the amino terminus of OPN of human, origin

· species reactivity for OPN of human,

· use for western blotting elisa,immunoprecipitation

and immunohistochemisty

Quantity size : 1ml （Reconstitued with 500ul pH 7.4 0.01 M PBS or diluent of antibody）

Application: · Western blotting： 1：200-500      

· ELISA： 1：500-1000

· Immunohistochemistry ：1：200-500

· Optimal working dilutions must be determined by the end user.

Storage:      Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles.

The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01 M PBS or diluent of antibody to 0.2ml, the antibody is stable for at least six weeks at 2-4 ºC.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.